Serum snoRNAs as biomarkers for joint ageing and post traumatic osteoarthritis

Sci Rep. 2017 Mar 2:7:43558. doi: 10.1038/srep43558.

Abstract

The development of effective treatments for the age-related disease osteoarthritis and the ability to predict disease progression has been hampered by the lack of biomarkers able to demonstrate the course of the disease. Profiling the expression patterns of small nucleolar RNAs (snoRNAs) in joint ageing and OA may provide diagnostic biomarkers and therapeutic targets. This study determined expression patterns of snoRNAs in joint ageing and OA and examined them as potential biomarkers. Using SnoRNASeq and real-time quantitative PCR (qRT-PCR) we demonstrate snoRNA expression levels in murine ageing and OA joints and serum for the first time. SnoRNASeq identified differential expression (DE) of 6 snoRNAs in young versus old joints and 5 snoRNAs in old sham versus old experimental osteoarthritic joints. In serum we found differential presence of 27 snoRNAs in young versus old serum and 18 snoRNAs in old sham versus old experimental osteoarthritic serum. Confirmatory qRT-PCR analysis demonstrated good correlation with SnoRNASeq findings. Profiling the expression patterns of snoRNAs is the initial step in determining their functional significance in ageing and osteoarthritis, and provides potential diagnostic biomarkers and therapeutic targets. Our results establish snoRNAs as novel markers of musculoskeletal ageing and osteoarthritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers
  • Cell-Free Nucleic Acids*
  • Gene Expression Profiling
  • Horses
  • Joints* / pathology
  • Male
  • Mice
  • Osteoarthritis / blood*
  • Osteoarthritis / etiology*
  • RNA, Small Nucleolar / blood
  • RNA, Small Nucleolar / genetics*
  • Real-Time Polymerase Chain Reaction
  • Reproducibility of Results
  • Transcriptome

Substances

  • Biomarkers
  • Cell-Free Nucleic Acids
  • RNA, Small Nucleolar